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KMID : 1149220020100020213
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Korean Journal of Oriental Medical Prescription
2002 Volume.10 No. 2 p.213 ~ p.223
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Apoptosis of 4-Acetyl-12, 13-epoxyl-9-trichothecene-3, 15-diol isolated from the fruiting bodies of Isaria japonica Yasuda via Caspase-3 in Bladder cancer Cell line (NBT-II)
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Kim Hyeong-Jin
Jang Seon-Il
Oh Gi-Su
Hong K.H.
Kim Youn-Chul
Pae Hyeon-Ok
Yun Young-Gab
Chung Hun-Taeg
Kwon Tae-Oh
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Abstract
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The fruiting bodies of Isaria japonica have been traditionally used in Korea to treat cancer. An apoptosis-inducing compound, 4-Acetyl-12, 13-epoxyl -9-trichothecene-3, I5-diol (AETD), was isolated from the methanol extract of fruiting bodies of Isaria japonica Yasuda by bioassay -guided fractionation. The apoptosis of murine bladder cancer cell line (NBT-Ⅱ) by the compound was accessed by propidium iodide staining flow cytometric analysis, and apoptosis-inducing activity at IC50 concentration (5 nmol／L) was further confirmed by a nuclear morphological change, a ladder pattern of DNA fragmentation, and an activation of caspase-3. These results indicate that AETD induces apoptosis of NBT-Ⅱ cells via expression of caspase-3.
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KEYWORD
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Murine NBT-II cells, 4-Acetyl-12, 13-epoxyl-9-trichothecene-3, 15-diol(AETD), Caspase-3, Apoptosis
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